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For this purpose, primary hepatic cells (1 10 6 cells/well) were seeded in 24-well plates, incubated for 24 h in culture conditions and subsequently pre-treated with SAG (at 2.00 mM) followed by exposure to CCl4

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When it gets to the end of a strand, its not a perfect fit on the end (the mechanics of this is a little complicated) and needs a bit of scratch DNA at the end of the strand to successfully complete the DNA replication